Device

Part:BBa_K249022:Design

Designed by: Roxanne Shank   Group: iGEM09_Lethbridge   (2009-08-26)


Target N-terminal CFP into Lumazine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

point mutations to remove EcoRI and PstI cut sites


Source

existing BioBrick parts, and synthesized DNA

References